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anchored oligo dt  (Integrated DNA Technologies)


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    Integrated DNA Technologies anchored oligo dt
    Anchored Oligo Dt, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 94/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anchored+oligo+dt/custom-51-01-15-08-41824445?v=Integrated+DNA+Technologies
    Average 94 stars, based on 56 article reviews
    anchored oligo dt - by Bioz Stars, 2026-07
    94/100 stars

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    List and characteristic features of oligonucleotide primers and probes used in this study. F: forward, R: reverse, PR: probe, RT: reverse transcription, Tm: melting temperature, RT-qPCR: singleplex/triplex RT-qPCR assays. 6-FAM: 6-Carboxyfluorescein; SUN: a fluorophore with a similar spectrum as HEX and VIC dyes. Cy5: Cyanine 5/IABkFQ/3′ Iowa Black ® FQ quencher; /IAbRQSp/3′ Iowa Black ® RQ quencher. ZEN, TAO: internal quenchers. 3′RACE: 3′ Rapid Amplification of cDNA ends. sn-PCR: semi-nested PCR, *: 3′RACE-snPCR product lengths were calculated with Adapter-1 as a reverse primer. # The primer is used only for reverse transcription reactions.
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    List and characteristic features of oligonucleotide primers and probes used in this study. F: forward, R: reverse, PR: probe, RT: reverse transcription, Tm: melting temperature, RT-qPCR: singleplex/triplex RT-qPCR assays. 6-FAM: 6-Carboxyfluorescein; SUN: a fluorophore with a similar spectrum as HEX and VIC dyes. Cy5: Cyanine 5/IABkFQ/3′ Iowa Black ® FQ quencher; /IAbRQSp/3′ Iowa Black ® RQ quencher. ZEN, TAO: internal quenchers. 3′RACE: 3′ Rapid Amplification of cDNA ends. sn-PCR: semi-nested PCR, *: 3′RACE-snPCR product lengths were calculated with Adapter-1 as a reverse primer. # The primer is used only for reverse transcription reactions.

    Journal: Viruses

    Article Title: Multiple Co-Infecting Caliciviruses in Oral Fluid and Enteric Samples of Swine Detected by a Novel RT-qPCR Assay and a 3′RACE-PCR-NGS Method

    doi: 10.3390/v17020193

    Figure Lengend Snippet: List and characteristic features of oligonucleotide primers and probes used in this study. F: forward, R: reverse, PR: probe, RT: reverse transcription, Tm: melting temperature, RT-qPCR: singleplex/triplex RT-qPCR assays. 6-FAM: 6-Carboxyfluorescein; SUN: a fluorophore with a similar spectrum as HEX and VIC dyes. Cy5: Cyanine 5/IABkFQ/3′ Iowa Black ® FQ quencher; /IAbRQSp/3′ Iowa Black ® RQ quencher. ZEN, TAO: internal quenchers. 3′RACE: 3′ Rapid Amplification of cDNA ends. sn-PCR: semi-nested PCR, *: 3′RACE-snPCR product lengths were calculated with Adapter-1 as a reverse primer. # The primer is used only for reverse transcription reactions.

    Article Snippet: For 3′ Rapid Amplification of cDNA ends in semi-nested RT-PCR (3′RACE-snPCR) reactions 5-5 μL of total RNA was converted to cDNA using Oligo dT-Anchor-Adapter primer ( ) and MAXIMA H-minus RT enzyme (Thermo-Fisher, Waltham, MA, USA) in a reverse transcription (RT) reaction in the total volume of 20 μL according to the manufacturer’s instructions.

    Techniques: Reverse Transcription, Rapid Amplification of cDNA Ends, Sequencing